WebApr 20, 2024 · Fig. 1 Strategy for obtaining multifunctional streptavidin–biotin conjugates with defined stoichiometries (SA 3 B 1, SA 2 B 2, SA 1 B 3) using the Ibio-His-Tag (iminobiotin-His-tag).The statistical mixture of the four streptavidin conjugates with different numbers of Ibio-His-Tags and the biotin-conjugated molecule, A, can be … WebMonoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH. Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol. …
biotin Etymology, origin and meaning of biotin by etymonline
WebJun 14, 2007 · The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in ... WebAnti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western ... the paralegals handbook
Using Avi-tag Biotinylated Proteins to Determine Protein …
WebCys-Streptavidin is a recombinant streptavidin for site-specific conjugation. Like the native streptavidin, Cys-Streptavidin has four identical subunits and binds specifically to biotin with an extremely high affinity. However, the subunit of the Cys-Streptavidin protein has a single cysteine residue at N-terminus for direct conjugation. WebPVDF membrane was probed with 0.1 µg/mL of Mouse Anti-His Tag Biotinylated Monoclonal Antibody (Catalog # BAM050) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using … WebAntibodies that detect 6x His Tag can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Immunohistochemistry. Our 6x His Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Goat, Sheep … shuttle from seatac airport